Fibrillarin Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis of Fibrillarin on different cell lysates using anti-Fibrillarin antibody at 1/1,000 dilution.
Lane 1: Hela
Lane 2: HepG2
Lane 3: 293T
Lane 4: NIH-3T3
Fig2: Flow cytometric analysis of HL-60 cells with Fibrillarin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Host Species; Species ReactivityRabbit; Human, Mouse
ImmunogenSynthetic Peptide of C-terminal human Fibrillarin.
Purification; FormulationPeptide affinity purified.; 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesrRNA 2'-O-methyltransferase fibrillarin, 34 kDa nucleolar scleroderma antigen, Histone-glutamine methyltransferase
BackgroundFibrillarin is a widely occurring, basic, nonhistone protein that is localized exclusively in the fibrillar region of the nucleolus, including both the dense fibrillar and the fibrillar center regions. Fibrillarin is also expressed in HeLa cells, 3T3 cells, and human peripheral blood lymphocytes. In metaphase and anaphase, fibrillarin is found on putative chromosomal nucleolar regions (NORs). During telophase, fibrillarin is an early marker for the site of the newly forming nucleolus. The structure of fibrillarin includes an RNA-binding domain and an RNP consensus sequence, which is consistent with the association of fibrillarin with the U3 small nucleolar RNA. Fibrillarin is involved in processing rRNA transcripts in the nucleolus.(ER1802-81)