FBXL2 Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis of FBXL2 on different lysates using anti-FBXL2 antibody at 1/1,000 dilution.
Lane 1: Mouse liver tissue Lane 2: Mouse heart tissue Lane 3: SiHa
Fig2: Western blot analysis of FBXL2 on rat liver tissue lysate using anti-FBXL2 antibody at 1/10,000 dilution.
Fig3: ICC staining FBXL2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Application SummaryWB, ICC, IHC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesF-box/LRR-repeat protein 2, F-box and leucine-rich repeat protein 2, F-box protein FBL2/FBL3
BackgroundCalcium-activated substrate recognition component of a SCF (SKP1-cullin-F-box protein) E3 ubiquitin-protein ligase complex which mediates the ubiquitination and subsequent proteasomal degradation of target proteins. Unlike many F-box proteins, FBXL2 doesn't seem to target phosphodegron within its substrates but rather calmodulin-binding motifs. Targets PCYT1A for its monoubiquitination and degradation, this is antagonized by calmodulin (By similarity). Targets the cyclins CCND2 and CCND3 for polyubiquitination and degradation, leading to cell-cycle arrest in G(0), also antagonized by calmodulin. Binds to hepatitis C virus non-structural protein 5A (NS5A) in a reaction crucial for hepatitis C virus RNA replication.(R1706-15)