ERK1 Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis of ERK1 on different cell lysates using anti- ERK1 antibody at 1/1000 dilution.
Lane 1: Hela
Lane 2: SW480
Lane 3: HCT116
Lane 4: HepG2
Lane 5: PC12
Fig2: ICC staining of ERK1 in LOVO cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon carcinoma tissue using anti-ERK1 antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Application SummaryWB, ICC, IHC
Purification; FormulationPeptide affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesMitogen-activated protein kinase 3,ERT2,Extracellular signal-regulated kinase 1，Insulin-stimulated MAP2 kinase，MAP kinase isoform p44，Microtubule-associated protein 2 kinase，p44-ERK1
BackgroundMitogen-activated protein kinases (MAPKs) are a widely conserved family of serine/threonine protein kinases involved in many cellular programs, such as cell proliferation, differentiation, motility, and death. The p44/42 MAPK (Erk1/2) signaling pathway can be activated in response to a diverse range of extracellular stimuli including mitogens, growth factors, and cytokines , and research investigators consider it an important target in the diagnosis and treatment of cancer . Upon stimulation, a sequential three-part protein kinase cascade is initiated, consisting of a MAP kinase kinase kinase (MAPKKK or MAP3K), a MAP kinase kinase (MAPKK or MAP2K), and a MAP kinase (MAPK). Multiple p44/42 MAP3Ks have been identified, including members of the Raf family, as well as Mos and Tpl2/COT. MEK1 and MEK2 are the primary MAPKKs in this pathway.(ER131011)