EP-CAM Mouse monoclonal Antibody IgM
Fig1: Western blot analysis of EpCAM on different cell lysates using anti- EpCAM antibody at 1/1000 dilution.
Lane 1: Hela
Lane 2: NIH/3T3
Lane 3: MCF-7
Fig2: ICC staining EpCAM in D3 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining EpCAM in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityMouse; Human, Mouse
Application SummaryWB, ICC, IHC, FC
Purification; FormulationProL affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesEpithelial cell adhesion molecule,Adenocarcinoma-associated antigen,Cell surface glycoprotein Trop-1,Epithelial cell surface antigen,Epithelial glycoprotein,Epithelial glycoprotein 314,KS 1/4 antigen,KSA,Major gastrointestinal tumor-associated protein GA733-2,Tumor-associated calcium signal transducer 1,CD_antigen: CD326
BackgroundEpithelial cell adhesion molecule (EpCAM) is a transmembrane glycoprotein mediating Ca2+-independent homotypic cell-cell adhesion in epithelia. EpCAM is also involved in cell signaling, migration, proliferation, and differentiation. Additionally, EpCAM has oncogenic potential via its capacity to upregulate c-myc, e-fabp, and cyclins A & E. Since EpCAM is expressed exclusively in epithelia and epithelial-derived neoplasms, EpCAM can be used as diagnostic marker for various cancers. It appears to play a role in tumorigenesis and metastasis of carcinomas, so it can also act as a potential prognostic marker and as a potential target for immunotherapeutic strategies. Mutations in EpCAM have also been associated with congenital tufting enteropathy which causes intractable diarrhea in newborn children. Specificity/Source: This antibody is produced by immunizing mice with recombinant protein corresponding to a region of EpCAM.(EM1111)