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EGFR Rabbit polyclonal Antibody IgG

SKU: BA110247-100µl
$279.00Price

Fig1: Western blot analysis of EGFR on different cell lysate using anti-EGFR antibody at 1/1,000 dilution.

Positive control:

Lane 1:  Hela

Lane 2:  HUVEC

Lane 3:  A431

Fig2: ICC staining EGFR in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-EGFR antibody. Counter stained with hematoxylin.

Bon Opus Cat. #BA110247
Size
  • Host Species; Species Reactivity

    Rabbit; Human
  • Immunogen

    Peptide
  • Application Summary

    WB, ICC, IHC
  • Purification; Formulation

    Peptide affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Epidermal growth factor receptor,Proto-oncogene c-ErbB-1,Receptor tyrosine-protein kinase erbB-1
  • Background

    The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Exons in the EGFR gene product are frequently either deleted or duplicated to produce deletion mutants (DM) or tandem duplication mutants (TDM), respectively, which are detected at various molecular weights. EGFR binds several ligands, including epidermal growth factor (EGF), transforming growth factor α (TGFα), Amphiregulin and heparin binding-EGF (HB-EGF). Ligand binding promotes the internalization of EGFR via Clathrin-coated pits and its subsequent degradation in response to its intrinsic tyrosine kinase. EGFR is involved in organ morphogenesis and maintenance and repair of tissues, but upregulation of EGFR is associated with tumor progression. The oncogenic effects of EGFR include initiation of DNA synthesis, enhanced cell growth, invasion and metastasis. Abrogation of EGFR results in cell cycle arrest, apoptosis or dedifferentiation of cancer cells, suggesting that EGFR may be an effective therapeutic target.(R1511-18)