EB3 Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of EB3 on different lysates using anti-EB3 antibody at 1/500 dilution.
Lane 1: Mouse brain tissue
Lane 2: K562
Fig2: ICC staining EB3 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining EB3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesMicrotubule-associated protein RP/EB family member 3, EB1 protein family member 3, End-binding protein 3, RP3
BackgroundEB1 (MAPRE2, microtubule-associated protein, RP/EB family, member 2, EB2, RP1) may influence tumorigenesis of colorectal cancers and proliferative control of normal cells. EB1 may belong to the intermediate/early gene family, involved in the signal transduction cascade downstream of the TCR. Colorectal cancer is caused by the pathologic transformation of normal colonic epithelium to an adenomatous polyp, which can become an invasive cancer. APC (adenomatous polyposis coli) is a tumor suppressor gene, the mutation of which is one of the earliest events in colorectal carcinogenesis. A majority of the mutations result in the loss of the carboxy terminus of APC. EB1 has been shown to bind to the carboxy terminal region of APC, which implicates EB1 in APC suppression of colonic cancer. EB1 overexpression may play a role in the development of human esophageal squamous cell carcinoma (ESCC) by affecting APC function and activating the beta-catenin/TCF pathway. EB3 is related to EB1 and likewise associates with the microtubule cytoskeleton. EB3 is expressed predominantly in the central nervous system and preferentially associates with APCL.(ET7106-99)