E-Cadherin Rabbit polyclonal Antibody IgG

SKU: BA110239-100µl
$279.00Price

Fig1: Western blot analysis of E-Cadherin on A431 cell lysate. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody was used at a 1:500 dilution in 5% BSA at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1:5,000 dilution was used for 1 hour at room temperature.

Fig2: ICC staining E-Cadherin in HCT116 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0407-25) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.

Fig3: ICC staining E-Cadherin in SKOV-3 cells (green). Formalin fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 10 minutes at room temperature and blocked with 1% Blocker BSA for 15 minutes at room temperature. Cells were probed with the antibody (0407-25) at a dilution of 1:200 for 1 hour at room temperature, washed with PBS. Alexa Fluorc™ 488 Goat anti-Rabbit IgG was used as the secondary antibody at 1/100 dilution.

Bon Opus Cat. #BA110239
Size
  • Host Species; Species Reactivity

    Rabbit; Human, Mouse
  • Immunogen

    Peptide
  • Application Summary

    WB, ICC, FC
  • Purification; Formulation

    Immunogen affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Cadherin-1,ARC-1,Epithelial cadherin,Uvomorulin,CD_antigen: CD324
  • Background

    Cadherins are calcium-dependent cell adhesion proteins. The classic cadherin subfamily includes N-, P-, R-, B- and E-cadherins as well as about ten other members which are found in adherens junctions (AJ), E-cadherin is found at the junctions of epithelial cells. Besides mediating cell-cell adhesion through homophilic interactions, E-cadherin mediates contact inhibition of cell growth, and loss of E-cadherin is associated with tumorigenesis. E-cadherin expression is frequently down-regulated or extinguished in malignancy which strongly correlates with poor prognosis.(0407-25)

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