DUSP1 Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of DUSP1 on mouse spleen lysates using anti-DUSP1 antibody at 1/1,000 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-DUSP1 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-DUSP1 antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Application SummaryWB, IHC, IP, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesDual specificity protein phosphatase 1, Dual specificity protein phosphatase hVH1, Mitogen-activated protein kinase phosphatase 1, Protein-tyrosine phosphatase CL100
BackgroundA key element in the pathway involved in the transduction of signals from activated protein-tyrosine kinase transmembrane receptors has been identified as the family of mitogen-activated protein kinases (MAP kinases). The most well known of these Ser/Thr kinases are ERK 1 and ERK 2. Mitogenic stimulation of cells triggers the activation of MAP kinases through phosphorylation of both tyrosyl (Y185) and threonyl (T183) residues. Phosphorylation of the T183 and Y185 ERK regulatory site is mediated by MAP kinase (MEK), which in turn is regulated by the proto-oncogene product Raf. Two highly related phosphatases, designated MKP-1 and MKP-2, exhibit 59% sequence identity at the amino acid level and oppose the action of MEK by downregulating the kinase activity of ERK 1 and ERK 2. MAP kinase phosphatase-1 and -2 proteins function by dephosphorylating ERK 1 and ERK 2 at their T-E-Y regulatory motif. An additional phosphatase encoded by the DUSP2 gene, designated PAC-1, also functions to downregulate ERK 1 and ERK 2 kinase activity. PAC-1 is a nuclear protein whose expression is strongly induced in response to mitogen.(ET1701-82)