Dnmt3a Recombinant Rabbit monoclonal Antibody IgG

Dnmt3a Recombinant Rabbit monoclonal Antibody IgG

SKU: BA111558-100µl
$279.00Price

Fig1: Western blot analysis of Dnmt3a on different lysates using anti-Dnmt3a antibody at 1/1,000 dilution.

Positive control:   

Lane 1: Hela            

Lane 2: Human brain 

Lane 3: Human heart

Fig2: ICC staining Dnmt3a in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining Dnmt3a in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bon Opus Cat. #BA111558
Size
  • Host Species; Species Reactivity

    Rabbit; Human, Rat
  • Immunogen

    Recombinant protein
  • Application Summary

    WB, ICC/IF
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    DNA (cytosine-5)-methyltransferase 3A, DNA methyltransferase HsaIIIA
  • Background

    Methylation at the 5'-position of cytosine is the only known naturally occurring covalent modification of the mammalian genome. DNA methylation requires the enzymatic activity of DNA 5-cytosine methyltransferase (Dnmt) proteins, which catalyze the transfer of a methyl group from S-adenosyl methionine to the 5'-position of cytosines residing in the dinucleotide CpG motif, and this methylation results in transcriptional repression of the target gene. The Dnmt enzymes are encoded by independent genes. Dnmt1 is the most abundant, and it preferentially methylates hemimethylated DNA and coordinates gene expression during development. Additional mammalian Dnmt proteins include Dnmt2 and Dnmt3. Dnmt2 lacks the large N-terminal regulator domain of Dnmt1, is expressed at substantially lower levels in adult tissues, and is likely involved in methylating newly integrated retroviral DNA. Dnmt3a and Dnmt3b are encoded by two distinct genes, but both are abundantly expressed in embryonic stem cells, where they also methylate CpG motifs on DNA.(ET1609-31)

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