DAPK3 Mouse monoclonal Antibody IgG1
Fig1: Western blot analysis of ZIP Kinase on human ZIP Kinase recombinant protein using anti- ZIP Kinase antibody at 1/1,000 dilution.
Fig2: Western blot analysis of ZIP Kinase on HEK293 (1) and ZIP Kinase -hIgGFc transfected HEK293 (2) cell lysate using anti- ZIP Kinase antibody at 1/1,000 dilution.
Fig3: Western blot analysis of ZIP Kinase on A431 (1) and K562 (2) cell lysate using anti- ZIP Kinase antibody at 1/1,000 dilution.
Host Species; Species ReactivityMouse; Human
Application SummaryWB, IHC, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesTranscription factor AP-2-alpha, AP-2 transcription factor, Activating enhancer-binding protein 2-alpha, Activator protein 2
BackgroundDAP (death associated protein) kinase and ZIP kinase are members of a novel protein kinase family, the members of which have the capacity to mediate apoptosis through their catalytic activities. DAP kinase contains a "death domain" and has been shown to mediate g interferon-induced apoptosis. The introduction of DAP kinase into highly metastatic carcinoma clones lacking DAP kinase expression was shown to result in the suppression of metastasis, thus linking suppression of apoptosis to metastasis. ZIP kinase contains a leucine zipper domain, which is necessary for homodimerization and for interaction with other leucine zipper proteins. ZIP kinase dimerizes with ATF-4, an ATF/CREB transcription factor family member that contains a leucine zipper. Overexpression of ZIP kinase was shown to result in morphological changes associated with apoptosis in NIH/3T3 cells.(EM1706-47)