DAP12 Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of DAP12 on THP-1 cell using anti-DAP12 antibody at 1/500 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-DAP12 antibody. Counter stained with hematoxylin.
Fig3: Flow cytometric analysis of K562 cells with DAP12 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Host Species; Species ReactivityRabbit; Human
ImmunogenSynthetic Peptide within human DAP12 aa 60-100.
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesTYRO protein tyrosine kinase-binding protein, DNAX-activation protein 12, Killer-activating receptor-associated protein
BackgroundNatural killer (NK) cells are regulated by stimulatory and inhibitory signals from a variety of receptors. Three main receptor families are responsible for NK cells recognition of MHC I molecules, including Ly-49, CD94/NKG2 and KIR (killer-cell inhibitory receptor). DAP12 is a phosphoprotein that is involved in the activation of NK cells. This protein interacts with membrane glycoproteins of the KIR family, resulting in cellular activation. DAP12 also binds to CD94/NKG2C, an activating NK cell receptor belonging to the C-type lectin superfamily. Additional proteins that bind to DAP12 include Ly-49D and Ly-49H, which associate with DAP12 in the plasma membrane. Phosphorylated DAP12 binds to ZAP-70 and Syk, suggesting that the activation pathway may be similar to that of the T and B cell antigen receptors.(ET7108-76)