Cyclin D1 Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis of cyclin D1 on mouse brain tissue lysates using anti-Cyclin D1 antibody at 1/5000 dilution.
Positive control: mouse brain
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human liver carcinoma tissue using anti-Cyclin D1 antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Application SummaryWB, IHC
Purification; FormulationPeptide affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
BackgroundCyclins function as regulators of CDKs (Cyclin-dependent kinase). Different cyclins exhibit distinct expression and degradation patterns which contribute to the temporal coordination of each mitotic event. This cyclin forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. Cyclin D1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Mutations, amplification and overexpression of this gene, which alters cell cycle progression, are observed frequently in a variety of tumors and may contribute to tumorigenesis. Immunohistochemical staining of cyclin D1 antibodies is used to diagnose mantle cell lymphoma. Cyclin D1 has been found to be overexpressed in breast carcinoma. Its potential use as a biomarker was suggested.(ER0722)