Cyclin B1 Recombinant Rabbit monoclonal Antibody IgG

SKU: BA111507-100µl

Fig1: Western blot analysis of Cyclin B1 on different lysates using anti-Cyclin B1 antibody at 1/1,000 dilution.

Positive control:   

Lane 1: Hela              

Lane 2: Daudi 

Lane 3: K562

Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cyclin B1 antibody. Counter stained with hematoxylin.


Bon Opus Cat. #BA111507
  • Host Species; Species Reactivity

    Rabbit; Human
  • Immunogen

    Recombinant protein
  • Application Summary

  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    G2/mitotic-specific cyclin-B1
  • Background

    In eukaryotic cells, mitosis is initiated following the activation of a protein kinase known variously as maturation-promoting factor, M-phase specific histone kinase or M-phase kinase. This protein kinase is composed of a catalytic subunit (Cdc2), a regulatory subunit (cyclin B) and a low molecular weight subunit (p13-Suc 1). The Cdc/cyclin enzyme is subject to multiple levels of control, of which the regulation of the catalytic subunit by tyrosine phosphorylation is the best understood. Tyrosine phosphorylation inhibits the Cdc2/cyclin B enzyme; tyrosine dephosphorylation, occurring at the onset of mitosis, directly activates the pre-MPF complex. Evidence has established that B type cyclins not only act on M-phase regulatory subunits of the Cdc2 protein kinase, but also activate the Cdc25A and Cdc25B endogenous tyrosine phosphatase, of which Cdc2 is the physiological substrate. The specificity of this effect is shown by the inability of either cyclin A or cyclin D1 to display any such stimulation of Cdc25A or Cdc25B.(ET1608-27)

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