Cyclin B1 Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis on 293 cell lysates using anti-Cyclin B1 rabbit polyclonal antibodies.
Fig2: Immunofluorescent staining of F9 cells using anti- Cyclin B1 rabbit polyclonal antibody.
Fig3: Immunofluorescent staining of Hela cells using anti- Cyclin B1 rabbit polyclonal antibody.
Host Species; Species ReactivityRabbit; Human, Mouse
Application SummaryWB, ICC, IHC, FC
Purification; FormulationImmunogen affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
BackgroundCyclins are a family of proteins that activate specific cyclin-dependent kinases required for progression through the cell cycle. The entry of all eukaryotic cells into mitosis is regulated by activation of cdc2/cdk1 at the G2/M transition. Cyclin B1 is essential for the control of the cell cycle at the G2/M (mitosis) transition. It interacts with the CDC2 protein kinase to form a serine/threonine kinase holoenzyme complex also known as maturation promoting factor (MPF). The cyclin subunit imparts substrate specificity to the complex. Four cyclin B1 phosphorylation sites (Ser126, 128, 133, and 147) are located in the cytoplasmic retention signal (CRS) domain and are thought to regulate the translocation of cyclin B1 to the nucleus at the G2/M checkpoint, promoting nuclear accumulation and initiation of mitosis.(R1308-13)