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CRISPR-Cas9 SP Recombinant Rabbit monoclonal Antibody IgG

SKU: BA112187-100µl
$279.00Price

Fig1: Western blot analysis of CRISPR-Cas9 SP on 293 and 293T cells lysates transfected with CRISPR-Cas9 using anti- CRISPR-Cas9 SP antibody at 1/500 dilution.

 

 

Bon Opus Cat. #BA112187
Size
  • Host Species; Species Reactivity

    Rabbit; Streptococcus pyogenes
  • Immunogen

    Recombinant protein
  • Application Summary

    WB, ICC/IF, IHC, FC
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    CRISPR-associated endonuclease Cas9/Csn1, SpCas9, SpyCas9
  • Background

    CRISPR (clustered regularly interspaced short palindromic repeat) is an adaptive immune system that provides protection against mobile genetic elements (viruses, transposable elements and conjugative plasmids). CRISPR clusters contain spacers, sequences complementary to antecedent mobile elements, and target invading nucleic acids. CRISPR clusters are transcribed and processed into CRISPR RNA (crRNA) (Probable). In type II CRISPR systems correct processing of pre-crRNA requires a trans-encoded small RNA (tracrRNA), endogenous ribonuclease 3 (rnc) and this protein. The tracrRNA serves as a guide for ribonuclease 3-aided processing of pre-crRNA. Subsequently Cas9/crRNA/tracrRNA endonucleolytically cleaves linear or circular dsDNA target complementary to the spacer. The target strand not complementary to crRNA is first cut endonucleolytically, then trimmed by 3'-5' exonucleolytically. DNA-binding requires protein and both RNA species. Cas9 probably recognizes a short motif in the CRISPR repeat sequences (the PAM or protospacer adjacent motif) to help distinguish self versus nonself.(ET1703-85)