CRISPR-Cas9 Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis on CRISPR-Cas9 transfected 293 cells (+) and non transtected 293 cells (-).
Fig2: Immunofluorescence analysis of CRISPR-Cas9 transfected 293 cells. The cell expressing CRISPR-Cas9 can be detected using rabbit anti- CRISPR-Cas9 (1:500) followed by FITC (1:1000) goat anti-rabbit IgG.
Host Species; Species ReactivityRabbit
Application SummaryWB, ELISA, ICC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
BackgroundThe Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR) Type II system is currently the most commonly used RNA-Guided Endonuclease technology for genome engineering. There are two distinct components to this system: (1) a guide RNA and (2) an endonuclease, in this case the CRISPR associated (Cas) nuclease, Cas9. The gRNA/Cas9 complex is recruited to the target sequence by the base-pairing between the gRNA sequence and the complement to the target sequence in the genomic DNA. For successful binding of Cas9, the genomic target sequence must also contain the correct Protospacer Adjacent Motiff (PAM) sequence immediately following the target sequence.(R1504-1)