CLOCK Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis on different lysates using anti-CLOCK rabbit polyclonal antibody. Positive control:
Lane 1: PC-12
Lane 2: NIH/3T3
Lane 3: Hela
Lane 4: MCF-7
Lane 5: Mouse brain
Lane 6: Mouse kidney
Fig2: Immunocytochemical staining of PC-3M cells using anti-CLOCK rabbit polyclonal antibody.
Fig3: Immunocytochemical staining of SW480 cells using anti-CLOCK rabbit polyclonal antibody.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Application SummaryWB, ICC, IHC, FC
Purification; FormulationPeptide affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesCircadian locomoter output cycles protein kaput,Class E basic helix-loop-helix protein 8
BackgroundBiological timepieces called circadian clocks are responsible for the regulation of hormonal rhythms, sleep cycles and other behaviors. The superchiasmatic nucleus (SCN), which is located in the brain, was the first mammalian circadian clock to be discovered. Clock, a member of the basic-helix-loop-helix-PAS (bHLH-PAS) family of transcription factors, has also been identified as having circadian function. Mutations within the Clock gene have been shown to increase the length of the endogenous period and to cause a loss of rhythmicity of circadian oscillations. Clock contains a DNA-binding domain, a protein dimerization domain and a glutamine-rich C-terminal region, which indicates transactivation capabilities. It has been speculated that Clock may regulate circadian rhythmicity in combination with other proteins such as Per. Per is also a PAS-domain containing protein that exhibits circadian function. Highest expression of Clock is seen in the hypothalamus and the eye.(R1511-2)