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CHFR Rabbit polyclonal Antibody IgG

SKU: BA110177-100µl

Fig1: Western blot analysis on Hela cell lysates using anti-CHFR rabbit polyclonal antibodies.

Fig2: ICC staining CHFR in A431 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS and counterstained with DAPI in order to highlight the nucleus (blue).


Bon Opus Cat. #BA110177
  • Host Species; Species Reactivity

    Rabbit; Human
  • Immunogen

  • Application Summary

    WB, ICC
  • Purification; Formulation

    Immunogen affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    E3 ubiquitin-protein ligase CHFR,Checkpoint with forkhead and RING finger domains protein,RING finger protein 196
  • Background

    E3 ubiquitin-protein ligase (CHFR) that functions in the antephase checkpoint by actively delaying passage into mitosis in response to microtubule poisons. It acts in early prophase before chromosome condensation, when the centrosome move apart from each other along the periphery of the nucleus. CHFR is probably involved in signaling the presence of mitotic stress caused by microtubule poisons by mediating the 'Lys-48'-linked ubiquitination of target proteins, leading to their degradation by the proteasome. Marked reduction of CHFR expression was detected in primary tumors and decreased CHFR expression was linked to promoter hypermethylation. Restoration of CHFR expression by treatment with the microtubule stress agent nocodazole and the methyltransferase inhibitor 5-aza-2'-deoxycytidine has been reported.(R1310-3)