Cdk4 Mouse monoclonal Antibody IgG2c
Fig1: Western blot analysis of Cdk4 on Hela using anti-Cdk4 antibody at 1/1,000 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-Cdk4 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue using anti-Cdk4 antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityMouse; Human, Mouse, Rat
ImmunogenThis antibody is produced by immunizing mice with Recombinant protein corresponding to a region of Cdk4 (aa 80-320).
Application SummaryWB, IHC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesCyclin-dependent kinase 4,Cell division protein kinase 4,PSK-J3
BackgroundCell cycle progression is controlled in part by a family of cyclin proteins and cyclin dependent kinases (Cdks). Cdk proteins work in concert with the cyclins to phosphorylate key substrates involved in each phase of cell cycle progression . Another family of proteins, Cdk inhibitors, also plays a role in regulating the cell cycle by binding to cyclin-Cdk complexes and modulating their activity. Several Cdk proteins have been identified, including Cdk2-Cdk8, PCTAIRE-1-PCTAIRE-3, PITALRE and PITSLRE. Cdk4, in complex with D-type cyclins, is thought to regulate cell growth during the G1 phase of the cell cycle. This association with a D-type cyclin upregulates Cdk4 activity, whereas binding to the Cdk inhibitor p16 downregulates Cdk4 activity. Activation of the Cdk4-cyclin complexes requires phosphorylation on a single threonyl residue of Cdk4, catalyzed by a Cdk-activating protein (CAK).(M1505-5)