CD74 Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of CD74 on Raji cells lysates using anti-CD74 antibody at 1/1,000 dilution.
Fig2: ICC staining CD74 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining CD74 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityRabbit; Human
Application SummaryWB, ICC/IF, IHC, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesHLA class II histocompatibility antigen gamma chain, HLA-DR antigens-associated invariant chain, Ia antigen-associated invariant chain, p33, CD_antigen: CD74
BackgroundThe human histocompatibility leukocyte antigen (HLA) class II-associated invariant chain is composed of at least four polypeptides. One of these polypeptide chains is expressed as a membrane-bound subunit and has been designated CD74. The loading of peptide onto the class II MHC protein (MHC II) appears to be regulated by CD74, which associates with MHC II during its migration to the endosomal compartment, where class II binds pep-tide. CD74 is expressed by cells of both T lymphocyte and B lymphocyte lineages. In fact, CD74 is broadly expressed in normal B lymphocytes, regardless of their histocompatibility leukocyte antigen (HLA) phenotype, while a subset of peripheral T lymphocytes that are MHC II negative do not express CD74.(ET1702-51)