CD73 Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of CD73 on A431 cells lysates using anti-CD73 antibody at 1/500 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD73 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-CD73 antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human
Application SummaryWB, IHC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
BackgroundCD73 (also designated ecto-5'-nucleotidase, E5NT, NT, NT5, NTE, eN and eNT) is a glycosyl-phosphatidylinositol (GPI)-anchored adhesion protein that catalyzes the dephosphorylation of extracellular purine and pyrimidine nucleotides to their corresponding bioactive nucleosides. CD73 is a dimer of two identical subunits that depends on GPI to link with the external face of the plasma membrane. Similar to other GPI-anchored proteins, CD73 mediates co-stimulatory signals in T cell activation. CD73 has few structural variants, yet elicits diverse biological function through differential regulation in endothelial cells (EC), subpopulations of B and T cells, germinal center follicular dendritic cells and on thymic medullary reticular fibroblasts. For example, IgG mediated neutralization of CD73 interferes with lymphocyte adhesion to EC, and blocks aggregation of germinal center B cells and follicular dendritic cells. Furthermore, IgG-mediated targeting of lymphocyte CD73, but not of endothelial cell CD73, causes shedding of CD73 and tyrosine phosphorylation of proteins.(ET1703-41)