CD6 Mouse monoclonal Antibody IgG2b
Fig1: Western blot analysis of CD6 on human CD6 recombinant protein using anti-CD6 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of CD6 on HEK293 (1) and CD6-hIgGFc transfected HEK293 (2) cell lysate using anti-CD6 antibody at 1/1,000 dilution.
Fig3: ICC staining CD6 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityMouse; Human
Application SummaryWB, IHC, ICC, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.; Liquid form.
BackgroundCD6 is a type I transmembrane glycoprotein that is present on mature thymocytes, peripheral T cells and a subset of B cells. The CD6 glycoprotein is tyrosine phosphorylated during TCR-mediated T-cell activation and the size difference between the CD6 forms is due in part to differences in phosphorylation state. CD6 protein contains a 24-amino acid signal sequence, three extracellular "scavenger receptor cysteine-rich" (SRCR) domains, a membrane-spanning domain, and a 44-amino acid cytoplasmic domain. CD6 shows significant homology to CD5. CD6, which is also found in brain and B cell chronic lymphocytic leukemias, plays an important role in interactions of thymocytes with thymic epithelial cells. CD6 molecules can physically associate with the TCR/CD3 complex.(EM1706-29)