CD47 Mouse monoclonal Antibody IgG1
Fig1: Western blot analysis of CD47 on NIH-3T3 cell (1), Mouse brain (2), Hela cell (3) and K562 cell (4) lysates using anti-CD47 antibody at 1/500 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-CD47 antibody. Counter stained with hematoxylin.
Fig3: Flow cytometric analysis of SH-SY5Y cells with CD47 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti mouse IgG was used as the secondary antibody.
Host Species; Species ReactivityMouse; Human, Mouse
Purification; FormulationProG affinity purified.; 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesLeukocyte surface antigen CD47, Antigenic surface determinant protein OA3, Integrin-associated protein, Protein MER6, CD_antigen: CD47
BackgroundCD47 is an integral membrane protein that plays a role in the regulation of cation fluxes across cell membranes. Specifically, CD47 is involved in the increase in intracellular calcium concentration that occurs upon cell adhesion to the extracellular matrix. It is also a receptor for the C-terminal cell binding domain of thrombospondin (SIRP). CD47 is absent from Rh-null erythrocytes, but does play a role in cell adhesion in non-erythroid cells and may prevent premature elimination of erythrocytes. It may also be involved in membrane permeability changes following viral infection. CD47 is expressed on hemopoietic cells, epithelial cells, endothelial cells and fibroblasts and is strongly expressed in brain and mesenchymal cells.(EM1701-37)