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CD4 Rabbit polyclonal Antibody IgG

SKU: BA110181-100µl

Fig1: Western blot analysis of CD4 on mouse brain lysate using anti-CD4 antibody at 1/500 dilution.

Fig2: ICC staining CD4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: Flow cytometric analysis of Raji cells with CD4 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.

Bon Opus Cat. #BA110181
  • Host Species; Species Reactivity

    Rabbit; Mouse, Rat
  • Immunogen

    This antibody is produced by immunizing rabbits with a synthetic Peptide (KLH-coupled) corresponding to mouse CD4 aa 200-250.
  • Application Summary

    WB, ICC, FC
  • Purification; Formulation

    Immunogen affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    T-cell surface glycoprotein CD4,T-cell differentiation antigen L3T4,T-cell surface antigen T4/Leu-3,CD_antigen: CD4
  • Background

    The CD4 gene encodes the T4 antigen, a glycoprotein of approximately 55 kDa, expressed on the surface of helper T lymphocytes.CD4 antigens are members of the immunoglobulin supergene family and are implicated as associative recognition elements in major histocompatibility complex class II-restricted immune responses. On T-lymphocytes they define the helper/inducer subset. CD4 antigens also serve as interleukin-15 receptors and bind to the HIV receptors, binding directly to the HIV envelope protein GP120.(0108-6)