CD34 Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis of CD34 on different cell lysates using anti-CD34 antibody at 1/1000 dilution.
Lane 1: Human brain
Lane 2: Mouse brain
Lane 3: Mouse testis
Lane 4: Mouse thymus
Lane 5: Mouse spleen
Lane 7: TF-1
Lane 8: F9
Fig2: ICC staining CD34 in hES cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- CD34 rabbit polyclonal antibody.
Host Species; Species ReactivityRabbit; Human, zebrafish
Application SummaryWB, ICC, IHC,FC
Purification; FormulationImmunogen affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.
BackgroundCD34 is a heavily glycosylated type I transmembrane molecule, that can be phoshorylated by a variety of kinases including Protein kinase C and Tyrosine kinases. CD34 antigen is expressed on small vessel endothelial cells and tumors of epithelial origin. CD34 is possibly an adhesion molecule with a putative role in early hematopoiesis by mediating the attachment of stem cells to the bone marrow extracellular matrix or directly to stromal cells. It could act as a scaffold for the attachment of lineage specific glycans, allowing stem cells to bind to lectins expressed by stromal cells or other marrow components. So CD34 is considered to be ideal marker for identifying and quantifying hematopoietic progenitor stem cells. With interaction with CrkL, CD34 becomes a substrate for PKC and CD34 surface expression is associated with activation of PKC.(0108-2)