CD19 Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of CD19 on different lysates using anti-CD19 antibody at 1/1,000 dilution.
Lane 1: Rat brain
Lane 2: 293
Fig2: Flow cytometric analysis of Jurkat cells with CD19 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Application SummaryWB, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesB-lymphocyte antigen CD19, B-lymphocyte surface antigen B4, Differentiation antigen CD19, T-cell surface antigen Leu-12, CD_antigen: CD19
BackgroundCD19 is a transmembrane glycoprotein that contains two extracellular immunoglobulin-like domains. CD19 is selectively expressed on the cell surface of B-lymphocytes, where it activates intracellular signaling cascades involving both Ras and phosphatidylinositol 3-kinase pathways. Activation of CD19 results in cross-linking of the membrane protein immunoglobulin chains and the subsequent association with Src family protein tyrosine kinases (PTK). Expression of CD19 is continuous throughout B-cell development and through terminal differentiation of B-cells into plasma cells. CD19 forms functional complexes with B-lymphocyte surface proteins, including integrin b1, CD21 and CD81, which are involved in regulating B-cell development.(ET1702-74)