CD14 Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis on K562 cell lysates using anti-CD14 rabbit polyclonal antibody.
Fig2: ICC staining CD14 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Flow cytometric analysis of Raji cells with CD14 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Host Species; Species ReactivityRabbit; Human, Mouse
ImmunogenSynthetic Peptide (KLH-coupled) within human CD14 150-210 aa.
Application SummaryWB, ICC, FC
Purification; FormulationImmunogen affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesMonocyte differentiation antigen CD14,Myeloid cell-specific leucine-rich glycoprotein,CD_antigen: CD14
BackgroundCD14 is a glycophosphatidylinositol-anchored protein expressed on the surfaces of monocytes and plymorphonuclear leukocytes. It cooperates with MD-2 and TLR4 to mediate the innate immune response to bacterial lipopolysaccharide (LPS). CD14 acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response and up-regulates cell surface molecules, including adhesion molecules.(R1107-2)