CCDC47 Mouse monoclonal Antibody IgG1
Fig1: Western blot analysis of CCDC47 on recombinant protein using anti-CCDC47 antibody at 1/1,000 dilution.
Fig2: ICC staining CCDC47 in F9 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining CCDC47 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityMouse; Human, Mouse
Application SummaryICC, IHC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesCoiled-coil domain-containing protein 47
BackgroundCoiled-coil domain 47 (CCDC47) is a gene located on human chromosome 17, specifically locus 17q23.3 which encodes for the protein CCDC47. The gene has several aliases including GK001 and MSTP041. The protein itself contains coiled-coil domains, the SEEEED superfamily, a domain of unknown function (DUF1682) and a transmembrane domain. The function of the protein is unknown, but it has been proposed that CCDC47 is involved in calcium ion homeostasis and the endoplasmic reticulum overload response. In addition to the high levels of phosphorylation seen in CCDC47, three sulfination sites are predicted and conserved in mammals, reptiles and birds but not in fish, amphibians or invertebrates. Five potential sumoylation sites are also seen and conserved back to the bony fish. There is no glycosylation of the protein as it is not predicted to extend into the extracellular portion of the cell.(M1004-3)