CBFb Recombinant Rabbit monoclonal Antibody IgG
Fig1: Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-CBFb antibody. Counter stained with hematoxylin.
Fig2: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CBFb antibody. Counter stained with hematoxylin.
Fig3: Flow cytometric analysis of SiHa cells with CBFb antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.
Host Species; Species ReactivityRabbit; Human, Rat
ImmunogenRecombinant protein within human CBFb aa 1-200.
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesCore-binding factor subunit beta, Polyomavirus enhancer-binding protein 2 beta subunit, SL3-3 enhancer factor 1 subunit beta, SL3/AKV core-binding factor beta subunit
BackgroundThe transcription factor Polyomavirus enhancer binding protein 2 (PEBP2), also designated Osf2 (Osteoblast-specific transcription factor), CBFA1 (Core Binding Factor) and AML3 (Acute myeloid leukemia), is composed of two subunits, a and b, which are essential for the regulation of hematopoiesis and osteogenesis. The PEBP2a subunits, PEBP2aA, PEBP2aB and PEBP2aC, are encoded by three RUNX genes, all of which contain a 128-amino acid region homologous to the highly conserved Drosophila segmentation gene, Runt. This region is involved in DNA binding and heterodimerization with the regulatory b subunit, which facilitates DNA binding of the a subunit. Both subunits are required for in vivo function; the disruption of either gene results in a lack of definitive hematopoiesis followed by embryo death in utero due to hemorrhage in the central nervous system. The gene encoding PEBP2b is the target of chromosomal inversion 16 (p13;q22) with the smooth muscle myosin heavy chain, producing a chimeric gene, PEBP2b/CBFB-SMMHC, that is associated with human acute myeloid leukemia.(ET7108-87)