Calreticulin Mouse monoclonal Antibody IgG1
Fig1: Western blot analysis of Calreticulin on HL-60 (1) and A431 (2) cell lysates using anti-Calreticulin antibody at 1/1,000 dilution.
Fig2: ICC staining Calreticulin (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Calreticulin antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityMouse; Human
Purification; FormulationProG affinity purified.; 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesCalreticulin, CRP55, Calregulin, Endoplasmic reticulum resident protein 60, HACBP, grp60
BackgroundCalnexin and calregulin (also called calreticulin) are calcium-binding proteins that are localized to the endoplasmic reticulum, Calnexin to the membrane and calregulin to the lumen. Calnexin is a type I membrane protein that interacts with newly synthesized glycoproteins in the endoplasmic reticulum. It may play a role in assisting with protein assembly and in retaining unassembled protein subunits in the endoplasmic reticulum. Calregulin has both low- and high-affinity calcium-binding sites. Neither Calnexin nor calregulin contains the calcium-binding "E-F hand" motif found in calmodulins. Calnexin and calregulin are important for the maturation of glycoproteins in the endoplasmic reticulum and appear to bind many of the same proteins.(EM1701-61)