Bmi1 Mouse monoclonal Antibody IgG2a
Fig1: Western blot analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1/1,000 dilution.
Lane 1: 293T
Lane 2: Jurkat
Lane 3: Hela
Lane 4: MCF-7
Lane 5: HepG2
Lane 6: NIH/3T3
Lane 7: PC12
Lane 8: Mouse kidney
Lane 9: Human kidney
Lane 10: K562
Lane 11: Human brain
Fig2: ICC staining Bmi1 in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: ICC staining Bmi1 in Lovo cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityMouse; Human, Mouse, Rat
ImmunogenRecombinant protein within human Bmi1 full sequence.
Application SummaryWB, ICC, IHC, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesPolycomb complex protein BMI-1,Polycomb group RING finger protein 4,RING finger protein 51
BackgroundThe Bmi-1 was identified initially as an oncogene that cooperates with c-myc in the generation of B-cell lymphoma. It contributes to the maintenance of cell identity, stem cell self-renewal, cell cycle regulation, and oncogenesis by maintaining the silenced state of genes that promote cell lineage specification, cell death, and cell-cycle arrest.(EM20602)