beta Ⅱ Tubulin Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of beta Ⅱ Tubulin on different lysates using anti-beta Ⅱ Tubulin antibody at 1/1,000 dilution.
Lane 1: Mouse brain
Lane 2: Mouse kidney
Fig2: ICC staining beta Ⅱ Tubulin in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-beta Ⅱ Tubulin antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Application SummaryWB, ICC/IF, IHC, IP
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesTubulin beta-2B chain, Tubulin beta-2A chain, Tubulin beta class IIa, Tubulin beta-4B chain, Tubulin beta-2 chain, Tubulin beta-2C chain
BackgroundTubulin is a major cytoskeleton component that has five distinct forms, designated a, b, g, d and e tubulin. a and b tubulins form heterodimers which multimerize to form a microtubule filament. Multiple b Tubulin isoforms (b1, b2, b3, b4, b5, b6 and b8) have been characterized and are expressed in mammalian tissues. b1 and b4 are present throughout the cytosol, b2 is present in the nuclei and nucleoplasm, and b3 is a neuron-specific cytoskeletal protein. g Tubulin forms the gammasome, which is required for nucleating microtubule filaments at the centrosome. Both d Tubulin and e Tubulin are associated with the centrosome. d Tubulin is a homolog of the Chlamydomonas d Tubulin Uni3 and is found in association with the centrioles, whereas e Tubulin localizes to the pericentriolar material. e Tubulin exhibits a cell cycle-specific pattern of localization; first associating with only the older of the centrosomes in a newly duplicated pair, and later associating with both centrosomes.(ET1609-48)