beta I Tubulin Mouse monoclonal Antibody IgG

SKU: BA113129-100µl
$279.00Price

Fig1: Sample: 

  K562 Cell (Human) Lysate at 40 ug

  Jurkat Cell (Human) Lysate at 40 ug

  Primary: Anti- beta I Tubulin (EM2001-01) at 1/1000 dilution

  Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution

  Predicted band size: 50 kD

  Observed band size: 50 kD

 

Fig2: Paraformaldehyde-fixed, paraffin embedded (Rat brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (EM2001-01) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructions and DAB staining.

Fig3: Paraformaldehyde-fixed, paraffin embedded (Mouse brain); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37℃ for 30min; Antibody incubation with (beta I Tubulin) Monoclonal Antibody, Unconjugated (EM2001-01 5F7) at 1:400 overnight at 4℃, followed by operating according to SP Kit(Mouse) (sp-0024) instructionsand DAB staining.

Bon Opus Cat. #BA113129
Size
  • Host Species; Species Reactivity

    Mouse; Human, Mouse, Rat
  • Immunogen

    KLH conjugated synthetic Peptide derived from human beta I Tubulin
  • Application Summary

    WB, IHC-P, FC
  • Purification; Formulation

    affinity purified by Protein G .01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Background

    Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha chain (By similarity).(EM2001-01)

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