Beta-Catenin Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of Beta-Catenin on different cell lysates using anti- Beta-Catenin antibody at 1/1,000 dilution.
Lane 1: SW480
Lane 2: HT29
Lane 3: A431
Fig2: Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti- Beta-Catenin antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti- Beta-Catenin antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Application SummaryWB, ICC, IHC, IP
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesCatenin beta-1, Beta-catenin
Backgroundβ-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines.(ET1601-5)