Beta-Catenin Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis of Beta-catenin on different lysates using anti-Beta-catenin antibody at 1/1,000 dilution.
Lane 1: A431
Lane 2: SW480
Lane 3: HCT116
Lane 4: HT-29
Lane 5: Mouse colon
Lane 6: Mouse brain
Fig2: Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using anti-Beta-catenin antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Beta-catenin antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
ImmunogenSynthetic Peptide (KLH-coupled) witihin human Beta-catenin C terminal.
Application SummaryWB, IHC, FC
Purification; FormulationPeptide affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
Backgroundβ-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.(ER0805)