Beta-Catenin Mouse monoclonal Antibody IgG1
Fig1: Western blot analysis of beta-catenin on different cell lysates using anti- beta-catenin antibody at 1/2000 dilution.
Fig2: ICC staining Beta-Catenin in NCCIT cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Beta-Catenin antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityMouse; Human, Mouse, Rat
ImmunogenSynthetic Peptide (KLH-coupled) within human Beta-catenin aa 320-400.
Application SummaryWB, ICC, IHC, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
Backgroundβ-Catenin is a key downstream effector in the Wnt signaling pathway. It is implicated in two major biological processes in vertebrates: early embryonic development and tumorigenesis. Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription. Phosphorylation on Tyr-654 decreases CDH1 binding and enhances TBP binding. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-catenin protein levels and have been found in many tumor cell lines. In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes.(EM0306)