BAX Mouse monoclonal Antibody IgA
Fig1: Western blot analysis of Bax on different lysates using anti-BAX antibody at 1/500 dilution.
Lane 1: Raji
Lane 2: Mouse lymphoid tissue
Fig2: ICC staining BAX in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded human gallbladder tissue using BAX antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityMouse; Human, Mouse
ImmunogenThis antibody is produced by immunizing mice with a synthetic Peptide (KLH-coupled) corresponding to N-terminal BAX.
Application SummaryWB, ICC, IHC, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesApoptosis regulator BAX,Bcl-2-like protein 4
BackgroundBAX is a member of the Bcl-2 gene family. Apoptosis regulator BAX promotes apoptosis by binding to and antagonizing the Bcl-2 protein. In healthy mammalian cells, the majority of BAX is found in the cytosol, but upon initiation of apoptotic signaling, Bax undergoes a conformational shift. Upon induction of apoptosis, BAX becomes organelle membrane-associated, and in particular, mitochondrial membrane associated. The expression of BAX is upregulated by the tumor suppressor protein p53, and BAX has been shown to be involved in p53-mediated apoptosis. The p53 protein is a transcription factor that, when activated as part of the cell's response to stress, regulates many downstream target genes, including BAX.(EM1203)