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BAP31 Recombinant Rabbit monoclonal Antibody IgG

SKU: BA112615-100µl
$279.00Price

Fig1: Western blot analysis of BAP31 on different lysates using anti-BAP31 antibody at 1/1,000 dilution.

 Positive control:

 Lane 1: A431  

              Lane 2: SK-Br-3

 

Fig2: ICC staining BAP31 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining BAP31 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bon Opus Cat. #BA112615
Size
  • Host Species; Species Reactivity

    Rabbit; Human, Mouse
  • Immunogen

    Synthetic Peptide within the C-terminus of human BAP31.
  • Application Summary

    WB,ICC,IF,IHC,FC
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    B-cell receptor-associated protein 31, 6C6-AG tumor-associated antigen, Protein CDM, p28
  • Background

    BAP31, a human Bcl-2-interacting protein, is an integral membrane protein that is a component of a protein complex in the endoplasmic reticulum. This protein complex mechanically bridges an apoptosis-initiating caspase, like procaspase-8, with the anti-apoptotic regulator Bcl-2 or Bcl-XL. The cytosolic domain of BAP31 contains two identical caspase recognition sites, which are preferentially cleaved by initiator caspases, including caspase 8. Cleavage of BAP31 during apoptosis generates a p20 fragment, which remains integrated in the membrane and, when expressed ectopically, is a potent inducer of cell death. BAP31 cleavage is important for manifesting cytoplasmic apoptotic events associated with membrane fragmentation and in the cross talk between mitochondria and the endoplasmic reticulum during Fas- mediated apoptosis. The BAP31 gene is ubiquitously expressed in murine tissues and is located on the X chromosome in both mouse and human.(ET7108-54)