Atg12 Rabbit polyclonal Antibody IgG
Fig1: Western blot analysis of Atg12 on different lysates using anti-Atg12 antibody at 1/500 dilution.
Lane 1: A431
Lane 2: Human kidney tissue
Lane 3: 293T
Fig2: Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Atg12 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Atg12 antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human
ImmunogenRecombinant protein within human Atg12 aa 1-100.
Purification; FormulationProtein affinity purified. ; 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesUbiquitin-like protein ATG12, Autophagy-related protein 12
BackgroundAtg12 (autophagy-related protein 12), also known as APG12, APG12L, FBR93 or HAPG12, is a 140 amino acid protein that is ubiquitously expressed and belongs to the Atg12 family of proteins. Atg12 is a homolog of the yeast protein Apg12 that participates in autophagy. Autophagy is a membrane trafficking mechanism that delivers cytoplasmic cargo to the vacuole/lysosome for degradation and recycling. In yeast, autophagy requires a protein conjugation system consisting of Apg12 covalently bound at the carboxy terminal glycine to lysine 149 of Apg5. Similarly in humans, Atg12 is essential for autophagy and localizes to the cytoplasm where it is covalently bound to APG5, a conjugation reaction that requires APG7, Atg10 and ATP. The Atg12-APG5 conjugate functions as an important regulator of the autophagic process and is required for the change in membrane morphology and development of autophagosomes. Due to alternative splicing events, two Atg12 isoforms exist.(ER1802-82)