Apg3 Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of Apg3 on different lysates using anti-Apg3 antibody at 1/500 dilution.
Lane 1: Mouse testis tissue
Lane 2: K562
Lane 3: HL-60
Lane 4: Hela
Lane 5: Jurkat
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Apg3 antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human thyroid gland tissue using anti-Apg3 antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesUbiquitin-like-conjugating enzyme ATG3, Autophagy-related protein 3, Protein PC3-96
BackgroundAtg3 (autophagy-related protein 3), also known as APG3-like, hAPG3 or PC3-96, is an E2-like enzyme that localizes to the cytoplasm and is expressed in a variety of tissues with predominant levels found in kidney, placenta, liver, heart and skeletal muscle. Atg3 catalyzes the formation of the Atg8-phosphatidylethanolamine (Atg8-PE) conjugate, a reaction that is essential for autophagy (a cellular process that allows for the degradation of organelles and bulk cellular proteins). The process of forming the Atg8-PE conjugate begins with the removal of the C-terminal arginine residue of Atg8 by Atg4, a cysteine protease. The, now exposed, glycine residue is then activated by Atg7 and is then transferred to Atg3 for the final conjugation to PE. This last step can be accelerated by the presence of the Atg12-Atg5 conjugate which functions similarly to an E3 enzyme.(ET1706-29)