APE1 Rabbit polyclonal Antibody IgG

SKU: BA111080-100µl
$279.00Price

Fig1: Western blot analysis of APE1 on different lysates using anti-APE1 antibody at 1/1,000 dilution.

 Positive control:

 Lane 1: HL-60    

   Lane 2: Human skin tissue  

 Lane 3: MCF-7    

   Lane 4: Mouse placenta tissue

 

Fig2: ICC staining APE1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining APE1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bon Opus Cat. #BA111080
Size
  • Host Species; Species Reactivity

    Rabbit; Human, Mouse, Rat
  • Immunogen

    Recombinant protein with Human APE1 aa 1-250.
  • Application Summary

    WB,ICC,IHC,FC
  • Purification; Formulation

    Protein affinity purified. ; 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    DNA-(apurinic or apyrimidinic site) lyase, APEX nuclease, Apurinic-apyrimidinic endonuclease 1, REF-1, Redox factor-1, DNA-(apurinic or apyrimidinic site) lyase, mitochondrial
  • Background

    The role of transcription factors in the regulation of gene expression is well established. Although the activity of these factors can be regulated by phosphorylation, evidence has indicated regulation of DNA binding mediated by changes in reduction-oxidation (redox) status. Mutational analysis has identified a single conserved cysteine residue mapping within the DNA binding domains of Fos and Jun. Chemical oxidation or modification of this cysteine residue inhibits the DNA binding activity of Fos and Jun. A similar mode of regulation has been recently proposed for other nuclear transcription factors. Oxidation is reversible by these compounds or by a cellular redox/DNA repair protein identified originally as Ref-1 (redox factor 1). Ref-1 is identical to a previously characterized DNA repair enzyme designated HAP1, APE or APEX.(ER1802-49)

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