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Anti-sfGFP antibdody Rabbit polyclonal Antibody IgG

SKU: BA111132-100µl

Fig1: Western blot analysis of sfGFP on fusion protein using anti-sfGFP antibody at 1/1,000 dilution.



Bon Opus Cat. #BA111132
  • Host Species; Species Reactivity

  • Immunogen

    Synthetic Peptide RDHMVLHEYVNAAGITC linked to KLH.
  • Application Summary

  • Purification; Formulation

    Peptide affinity purified.; 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • Background

    Tagging proteins with a functional sequence is a powerful way to access function, and inserting tags at endogenous genomic loci allows the preservation of a near-native cellular background. GFP11 is the 11th beta-strand of the superfolder GFP beta-barrel structure. When expressed in the same cell, GFP11 and its complementary GFP fragment (GFP1-10) enable functional GFP tagging upon complementation. The key advantage of the GFP11 is its small size (16 aa): this allows commercial ssDNA oligomers to be used as HDR donors, circumventing any requirement for molecular cloning.(ER1802-92)