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AMPK gamma1 Mouse monoclonal Antibody IgG1

SKU: BA113134-100µl
$279.00Price

Fig1: Sample: 

  U87MG(Human) Cell Lysate at 30 ug

  Primary: Anti- AMPK gamma1 (EM2001-06) at 1/1000 dilution

  Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution

  Predicted band size: 36 kD

  Observed band size: 36 kD

 

 

 

Bon Opus Cat. #BA113134
Size
  • Host Species; Species Reactivity

    Mouse; Human
  • Immunogen

    Recombinant human AMPK gamma1 Protein
  • Application Summary

    WB
  • Purification; Formulation

    affinity purified by Protein A .01M TBS(pH7.4) with 1% BSA, 0.03% Proclin300 and 50% Glycerol.
  • Background

    AMP/ATP-binding subunit of AMP-activated protein kinase (AMPK), an energy sensor protein kinase that plays a key role in regulating cellular energy metabolism. In response to reduction of intracellular ATP levels, AMPK activates energy-producing pathways and inhibits energy-consuming processes: inhibits protein, carbohydrate and lipid biosynthesis, as well as cell growth and proliferation. AMPK acts via direct phosphorylation of metabolic enzymes, and by longer-term effects via phosphorylation of transcription regulators. Also acts as a regulator of cellular polarity by remodeling the actin cytoskeleton; probably by indirectly activating myosin. Gamma non-catalytic subunit mediates binding to AMP, ADP and ATP, leading to activate or inhibit AMPK: AMP-binding results in allosteric activation of alpha catalytic subunit (PRKAA1 or PRKAA2) both by inducing phosphorylation and preventing dephosphorylation of catalytic subunits. ADP also stimulates phosphorylation, without stimulating already phosphorylated catalytic subunit. ATP promotes dephosphorylation of catalytic subunit, rendering the AMPK enzyme inactive.(EM2001-06)