Alpha-tubulin Mouse monoclonal Antibody IgG3
Fig1: Western blot analysis on PC12 cell lysates using anti-α-tubulin mouse mAb.
Fig2: ICC staining α-tubulin in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Western blot analysis of α-tubulin on hybrid fish (crucian-carp) brain tissue lysate using anti-α-tubulin antibody at 1/500 dilution.
Host Species; Species ReactivityMouse; Human, Mouse, Rat, Zebrafish
Application SummaryWB, ICC, IHC, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesTubulin alpha-4A chain,Alpha-tubulin 1,Testis-specific alpha-tubulin,Tubulin H2-alpha,Tubulin alpha-1 chain
BackgroundThe cytoskeleton consists of three types of cytosolic fibers: microtubules, microfilaments (actin filaments), and intermediate filaments. Globular tubulin subunits comprise the microtubule building block, with α/β-tubulin heterodimers forming the tubulin subunit common to all eukaryotic cells. Acetylation of alpha chains at Lys-40 stabilizes microtubules and affects affinity and processivity of microtubule motors. This modification has a role in multiple cellular functions, ranging from cell motility, cell cycle progression or cell differentiation to intracellular trafficking and signaling.(M1501-1)