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Alpha smooth muscle Actin Rabbit polyclonal Antibody IgG

SKU: BA110034-100µl
$279.00Price

Fig1: Western blot analysis of alpha smooth muscle actin on different cell lysates using anti-Alpha smooth muscle actin antibody at 1/2000 dilution.

Positive control: 

  Lane 1: Jurkat 

  Lane2 : NIH/3T3 

  Lane 3: Mouse embryonic stem cell 

  Lane 4: Mouse colon

Fig2: ICC staining of Alpha smooth muscle actin in 293 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Fig3: ICC staining of Alpha smooth muscle actin in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Bon Opus Cat. #BA110034
Size
  • Host Species; Species Reactivity

    Rabbit; Human, Mouse, Rat(Predicted to react with Xenopus laevis, Chicken, Cow, Horse, Monkey)
  • Immunogen

    Peptide
  • Application Summary

    WB, ICC, IHC
  • Purification; Formulation

    Peptide affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Actin, cytoplasmic 1,Beta-actin
  • Background

    Actins are highly conserved proteins involved in cell motility, structure and integrity. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis.(ER1003)