Alpha smooth muscle Actin Mouse monoclonal Antibody IgG2b
Fig1: Western blot analysis of alpha smooth muscle Actin on NIH-3T3 (1), human heart (2) and mouse heart (3) using anti-alpha smooth muscle Actin antibody at 1/1,000 dilution.
Fig2: ICC staining alpha smooth muscle Actin (red) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig3: Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-alpha smooth muscle Actin antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityMouse; Human, Mouse, Rat
ImmunogenThis antibody is produced by immunizing mice with a synthetic Peptide (KLH-coupled) corresponding to a region of alpha smooth muscle Actin.
Application SummaryWB, ICC, IHC, FC
Purification; FormulationProG affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesActin, cytoplasmic 1,Beta-actin
BackgroundActins are highly conserved proteins involved in cell motility, structure and integrity. Actin has been found to be expressed in at least six isomeric forms. It is expressed in heart and skeletal striated muscle tissue, and in certain smooth muscle tissues, regulating contractile potentials for these cells. It is also expressed in the cytoplasm of non-muscle cells, functioning to control cell structure and motility. Smooth muscle alpha actin is of further interest because it is one of a few genes whose expression is relatively restricted to vascular smooth muscle cells. Expression of smooth muscle alpha actin is regulated by hormones, cell proliferation, and altered by pathological conditions including oncogenic transformation and atherosclerosis.(M1310-6)