AIM2 Mouse monoclonal Antibody IgG1
Fig1: Western blot analysis of AIM2 on human AIM2 recombinant protein using anti-AIM2 antibody at 1/1,000 dilution.
Fig2: Western blot analysis of AIM2 on HEK293 (1) and AIM2-hIgGFc transfected HEK293 (2) cell lysate using anti-AIM2 antibody at 1/1,000 dilution.
Fig3: ICC staining AIM2 (green) and Actin filaments (red) in A549 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityMouse; Human
Application SummaryWB, ICC, FC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesRetinoblastoma-like protein 2, 130 kDa retinoblastoma-associated protein, Retinoblastoma-related protein 2, pRb2
BackgroundInterferon-inducible protein AIM2 (Absent in melanoma 2) is a 343 amino acid protein belonging to the HIN-200 family. Induced by IFN-γ, AIM2 is thought to act as a tumor suppressor by repressing NFκB transcriptional activity. Localized to the nucleus, AIM2 contains one DAPIN domain and one HIN-200 domain. The DAPIN domain is composed mostly of α-helixes and is a protein-protein interaction domain capable of binding other DAPIN domains. The HIN-200 domain has been shown to bind directly to DNA, which, along with the binding of another protein ASC, results in the activation of Caspase-1. AIM2 is present as a homodimer and is expressed highly in small intestine, testis, peripheral blood leukocytes and spleen. Defects in AIM2 are believed to be a cause of microsatellite unstable colon cancers.(EM1706-42)