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active+pro Caspase 3 Recombinant Rabbit monoclonal Antibody IgG

SKU: BA111533-100µl
$279.00Price

Fig1: Western blot analysis of active+pro Caspase 3 on Jurkat cells lysates using anti-active+pro Caspase 3 antibody at 1/1,000 dilution.

Fig2: Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-active+pro Caspase 3 antibody. Counter stained with hematoxylin.

Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-active+pro Caspase 3 antibody. Counter stained with hematoxylin.

Bon Opus Cat. #BA111533
Size
  • Host Species; Species Reactivity

    Rabbit; Human
  • Immunogen

    Recombinant protein
  • Application Summary

    WB, IHC, IP
  • Purification; Formulation

    ProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
  • ALTnames

    Caspase-3, Apopain, Cysteine protease CPP32, Protein Yama, SREBP cleavage activity 1
  • Background

    Caspase-3, also known as apopain, SCA-1, Yama and CPP32, is an aspartate-specific cysteine protease that belongs to the ICE subfamily of caspases. Caspase-3 is expressed in cells as an inactive precursor from which the p17 and p11 subunits of the mature caspase-3 are proteolytically generated during apoptosis. The caspase-3 precursor is first cleaved at Asp175-Ser176 to produce the p11 subunit and the p20 peptide. Subsequently, the p20 peptide is cleaved at Asp28-Ser29 to generate the mature p17 subunit. The active caspase-3 enzyme is a heterodimer composed of two p17 and two p11 subunits. At the onset of apoptosis, caspase-3 proteolytically cleaves PARP at an Asp216-Gly217 bond. During the execution of the apoptotic cascade, activated caspase-3 releases SREBP from the membrane of the ER in a proteolytic reaction that is distinct from their normal sterol-dependent activation. Caspase-3 cleaves and activates SREBPs between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase-3 also cleaves and activates caspase-6, -7 and -9. The human caspase-3 gene encodes a cytoplasmic protein that is highly expressed in lung, spleen, heart, liver, kidney and cells of the immune system.(ET1608-64)