Acetyl CoA synthetase Recombinant Rabbit monoclonal Antibody IgG
Fig1: ICC staining Acetyl CoA synthetase in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Fig2: ICC staining Acetyl CoA synthetase in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.
Host Species; Species ReactivityRabbit; Human, Mouse, Rat
Application SummaryWB, ICC/IF
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesAcetyl-coenzyme A synthetase, cytoplasmic, Acetate--CoA ligase, Acetyl-CoA synthetase, Acyl-CoA synthetase short-chain family member 2, Acyl-activating enzyme
BackgroundACSS2 (acyl-CoA synthetase short-chain family member 2), also known as ACAS2, ACS, ACSA or AceCS, is a 701 amino acid cytoplasmic protein that belongs to the ATP-dependent AMP-binding enzyme family. Existing as a monomer, ACSS2 functions to catalyze the ATP-dependent activation of acetate, a reaction that yields acetyl-CoA for use in energy generation and lipid synthesis. ACSS2 expression, which is highest in liver and kidney tissue, is regulated by the presence of unsaturated fatty acids and sterol regulatory element-binding proteins (SREBPs). Human ACSS2 exists as two alternatively spliced isoforms and shares 93% sequence identity with its mouse counterpart, suggesting a conserved role between species.(ET1702-21)