A2M Recombinant Rabbit monoclonal Antibody IgG
Fig1: Western blot analysis of A2M on human placenta cells lysates using anti-A2M antibody at 1/500 dilution.
Fig2: Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti- A2M antibody. Counter stained with hematoxylin.
Fig3: Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- A2M antibody. Counter stained with hematoxylin.
Host Species; Species ReactivityRabbit; Human
Application SummaryWB, IHC
Purification; FormulationProA affinity purified; 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide.; Liquid form.
ALTnamesAlpha-2-macroglobulin, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 5
Backgroundα-2-Macroglobulin (α-2M) is a homotetrameric serum protein consisting of four identical subunits that form dimers through disulfide bonds. Initially, α-2M was characterized as a pan-proteinase inhibitor that was able to "bait" proteinases into cleaving specific peptide sequences on α-2M. This interaction induces a conformational change in α-2M, thus enabling it to "trap" the proteinase and further inhibit its activity. Subsequently, α-2M has been shown to function as a carrier protein and regulator of cytokines during inflammation. Circulating transforming growth factor β (TGFβ) in serum is primarily bound to α-2M, which renders TGFβ inactive. α-2M also binds to IL-6 and, thereby, increases the concentration of IL-6 near lymphocytes, hepatocytes and stem cells involved in mediating the inflammatory cascade. Mutations and deletions in the gene encoding α-2M are associated with an increased incidence of Alzheimer's Disease (AD), which is consistent with the role of α-2M in mediating the clearance and degradation of A β, the major component of β-Amyloid deposits accumulated during AD.(ET1703-69)